{"id":219,"date":"2018-08-26T20:45:26","date_gmt":"2018-08-26T20:45:26","guid":{"rendered":"http:\/\/orobanche.net\/?p=219"},"modified":"2022-02-08T15:31:16","modified_gmt":"2022-02-08T15:31:16","slug":"fosfat-tamponu-nasil-hazirlanir","status":"publish","type":"post","link":"https:\/\/orobanche.net\/?p=219&lang=tr","title":{"rendered":"Fosfat Tamponu Nas\u0131l Haz\u0131rlan\u0131r ?"},"content":{"rendered":"

 <\/strong><\/p>\n

E\u011fer literat\u00fcrde ba\u015fka bir tampon kullanman\u0131z belirtilmemi\u015f ise enzim \u00e7al\u0131\u015fmalar\u0131nda Potasyum yada Sodyum fostat tamponlar\u0131n\u0131 kullanabilirsiniz. Suda kolayl\u0131kla \u00e7\u00f6z\u00fcn\u00fcrler. Ph de\u011fi\u015fkenlik g\u00f6sterdi\u011finden dolay\u0131 ayr\u0131 ayr\u0131 haz\u0131rlay\u0131p kendiniz istedi\u011finiz Ph’y\u0131 ayarlaman\u0131z daha iyi olacakt\u0131r.<\/p>\n

Potasyum fosfat\u0131n Monobazik ve Dibazik olarak iki ayr\u0131 formlar\u0131 bulunmaktad\u0131r. Haz\u0131rlamak i\u00e7in;<\/strong><\/p>\n

Dibazik potasyum fostat (K2<\/sub>HPO4<\/sub>) 1M molek\u00fcl a\u011f\u0131rl\u0131\u011f\u0131 : 174.18 gr<\/strong><\/p>\n

Monobazik potasyum fostat (KH2<\/sub>PO4<\/sub>) 1M molek\u00fcl a\u011f\u0131rl\u011f\u0131 : 136.09 gr d\u0131r.<\/strong><\/p>\n

\u0130\u00e7inde \u00e7\u00f6zebilmeniz i\u00e7in ddH2<\/sub>O (ultra saf su)<\/strong><\/p>\n

\u015eimdi \u00f6ncelikle 0,5 L her iki formundan stok haz\u0131rlaman\u0131z gerekmektedir.<\/p>\n

1. 87.09gr Dibazik potasyum fostat (K2<\/sub>HPO4<\/sub>) (174.18 gr olan) tart\u0131n ve 450 mL suya ekliyerek iyice \u00e7\u00f6z\u00fcn. Tamamen \u00e7\u00f6z\u00fcnd\u00fckten sonra oda \u015fartlar\u0131nda \u00fcst\u00fcne 0,5 L olacak \u015fekilde tamamlay\u0131n.<\/p>\n

2. 68.045 gr Monobazik potasyum fostat (KH2<\/sub>PO4<\/sub>) ( 136.09gr olan ) tart\u0131n ve 450 mL suya ekliyerek iyice \u00e7\u00f6z\u00fcn. Tamamen \u00e7\u00f6z\u00fcnd\u00fckten sonra oda \u015fartlar\u0131nda \u00fcst\u00fcne 0,5 L olacak \u015fekilde tamamlay\u0131n.<\/p>\n

3. Elimizdeki stoklar ile a\u015fa\u011f\u0131daki tablolar yard\u0131m\u0131yla 100mM yada 50mM l\u0131k istenilen pH\u2019da 1L distile su ile seyrelterek tampon elde etmeniz m\u00fcmk\u00fcnd\u00fcr.<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

Kaynak : https:\/\/openwetware.org\/wiki\/Lidstrom:Buffers#phosphate_buffer_basics<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

  E\u011fer literat\u00fcrde ba\u015fka bir tampon kullanman\u0131z belirtilmemi\u015f ise enzim \u00e7al\u0131\u015fmalar\u0131nda Potasyum yada Sodyum fostat tamponlar\u0131n\u0131 kullanabilirsiniz. Suda kolayl\u0131kla \u00e7\u00f6z\u00fcn\u00fcrler. Ph de\u011fi\u015fkenlik g\u00f6sterdi\u011finden dolay\u0131 ayr\u0131 ayr\u0131 haz\u0131rlay\u0131p kendiniz istedi\u011finiz Ph’y\u0131…<\/p>\n","protected":false},"author":1,"featured_media":1594,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"_links_to":"","_links_to_target":""},"categories":[9],"tags":[],"class_list":["post-219","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-genel-tr"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"https:\/\/orobanche.net\/wp-content\/uploads\/2018\/08\/pexels-photo-6208927.jpeg","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p9Bqz9-3x","jetpack_likes_enabled":true,"jetpack-related-posts":[{"id":296,"url":"https:\/\/orobanche.net\/?p=296&lang=tr","url_meta":{"origin":219,"position":0},"title":"Agaroz Jel Elektroforezi i\u00e7in tampon haz\u0131rlamak","author":"graiv","date":"Ocak 1, 2019","format":false,"excerpt":"Jel elekroforozi, negatif y\u00fckl\u00fc olan DNA par\u00e7ac\u0131klar\u0131n\u0131n agaroz jel \u00fczerinde eksi-art\u0131 kutbu boyunca hareket etmesi ve bu sayede molek\u00fcl a\u011f\u0131rl\u0131\u011f\u0131na g\u00f6re DNA par\u00e7alar\u0131n\u0131n ayr\u0131lmas\u0131nda kullan\u0131lan bir y\u00f6ntemdir (Tabi ki jel elektroforozi sadece DNA\u2019 n\u0131n ayr\u0131lmas\u0131nda kullan\u0131lmaz protein ayr\u0131lmas\u0131 gibi farkl\u0131 kullan\u0131m ama\u00e7lar\u0131 mevcuttur).\u00a0 Jel tank\u0131nda bu hareketi sa\u011flayacak bir iyonik\u2026","rel":"","context":""Akademik Yaz\u0131lar\u0131m" i\u00e7inde","block_context":{"text":"Akademik Yaz\u0131lar\u0131m","link":"https:\/\/orobanche.net\/?cat=9&lang=tr"},"img":{"alt_text":"woman in white laboratory gown","src":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/01\/pexels-photo-3735782.jpeg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/01\/pexels-photo-3735782.jpeg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/01\/pexels-photo-3735782.jpeg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/01\/pexels-photo-3735782.jpeg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/01\/pexels-photo-3735782.jpeg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/01\/pexels-photo-3735782.jpeg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":248,"url":"https:\/\/orobanche.net\/?p=248&lang=tr","url_meta":{"origin":219,"position":1},"title":"Enzim Aktivite \u00d6l\u00e7\u00fcmleri \u0130\u00e7in Ezme Tamponu Haz\u0131rlanmas\u0131","author":"graiv","date":"Eyl\u00fcl 9, 2018","format":false,"excerpt":"Ba\u015flamadan \u00f6nce her zaman dedi\u011fim gibi literat\u00fcre iyice bir bak\u0131n. Baz\u0131 durumlarda farkl\u0131 bitkiler i\u00e7in farkl\u0131 ezme tamponlar\u0131 kullan\u0131lmaktad\u0131r. Genel olarak benim kullanmd\u0131\u011f\u0131m ezme tamponunun haz\u0131rlanmas\u0131n\u0131 anlataca\u011f\u0131m. SOD, CAT, APX, GPX, POD ve PPO enzim aktivitelerini belirlemede ayn\u0131 tamponu kullanabilirsiniz. \u00d6ncelikle 100mM (pH 7,5) k-fosfat tamponunuz haz\u0131r bulunmal\u0131d\u0131r. Nas\u0131l haz\u0131rland\u0131\u011f\u0131n\u0131\u2026","rel":"","context":""Akademik Yaz\u0131lar\u0131m" i\u00e7inde","block_context":{"text":"Akademik Yaz\u0131lar\u0131m","link":"https:\/\/orobanche.net\/?cat=9&lang=tr"},"img":{"alt_text":"crop chemist pouring clear liquid into fragile glassware in science center","src":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2018\/09\/pexels-photo-3825379.jpeg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2018\/09\/pexels-photo-3825379.jpeg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2018\/09\/pexels-photo-3825379.jpeg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2018\/09\/pexels-photo-3825379.jpeg?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":1346,"url":"https:\/\/orobanche.net\/?p=1346&lang=tr","url_meta":{"origin":219,"position":2},"title":"Trizol Reagent Haz\u0131rlanmas\u0131","author":"graiv","date":"Mart 2, 2019","format":false,"excerpt":"\u00d6zellikle RNA izolasyonu i\u00e7in kullan\u0131l\u0131r. Trizol y\u00f6ntemi di\u011fer y\u00f6ntemler ile kar\u015f\u0131la\u015ft\u0131r\u0131ld\u0131\u011f\u0131nda KRAL d\u0131r. E\u011fer Trizol y\u00f6ntemi sizin bitki \u00f6rne\u011finizde kullan\u0131l\u0131yor ve \u00e7al\u0131\u015f\u0131yorsa kesinlikle kullan\u0131n derim. Tabi para olsa da \u0130zolasyon kitleri kullansak. Gerekli Malzemeler Sature edilmi\u015f Fenol (Folin ayrac\u0131 ile kar\u0131\u015ft\u0131rmay\u0131n. Sigma Kodu : Guanidine thiocyanate (Molek\u00fcl A\u011f\u0131rl\u0131\u011f\u0131 : 118,16\u2026","rel":"","context":""Akademik Yaz\u0131lar\u0131m" i\u00e7inde","block_context":{"text":"Akademik Yaz\u0131lar\u0131m","link":"https:\/\/orobanche.net\/?cat=9&lang=tr"},"img":{"alt_text":"crop chemist pouring clear liquid into fragile glassware in science center","src":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/03\/pexels-photo-3825379.jpeg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/03\/pexels-photo-3825379.jpeg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/03\/pexels-photo-3825379.jpeg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/03\/pexels-photo-3825379.jpeg?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":250,"url":"https:\/\/orobanche.net\/?p=250&lang=tr","url_meta":{"origin":219,"position":3},"title":"Molarite Hesaplama","author":"graiv","date":"Eyl\u00fcl 9, 2018","format":false,"excerpt":"En zorland\u0131\u011f\u0131m durumlardan biriydi labaratuvarda \u00e7al\u0131\u015f\u0131rken ka\u00e7 gram katmal\u0131y\u0131m sorusu. \u0130lk zamanlar makaleri okurken ve denemeye \u00e7al\u0131\u015f\u0131rken \u201c3 mL 100 mM k-phosphate buffer (pH7.8) containing 0,5 mM EDTA-Na 2 and 0.5 mM ascorbate.\u201d g\u00f6rd\u00fc\u011f\u00fcmde korkard\u0131m. Ka\u00e7 gr kat\u0131cam ki ka\u00e7 mol olacak diye tam bir karma\u015fa gelirdi. Asl\u0131nda litre \u00e7\u00f6z\u00fcc\u00fcdeki\u2026","rel":"","context":""Akademik Yaz\u0131lar\u0131m" i\u00e7inde","block_context":{"text":"Akademik Yaz\u0131lar\u0131m","link":"https:\/\/orobanche.net\/?cat=9&lang=tr"},"img":{"alt_text":"clever african american student displaying chemical formula on magnetic whiteboard","src":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2018\/09\/pexels-photo-3825457.jpeg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2018\/09\/pexels-photo-3825457.jpeg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2018\/09\/pexels-photo-3825457.jpeg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2018\/09\/pexels-photo-3825457.jpeg?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":1576,"url":"https:\/\/orobanche.net\/?p=1576&lang=tr","url_meta":{"origin":219,"position":4},"title":"CTAB ile Bitkilerden Y\u00fcksek Miktarda RNA \u0130zolasyonu","author":"graiv","date":"\u015eubat 8, 2022","format":false,"excerpt":"Gerekli Malzemeler CTAB ezme tamponu (Haz\u0131rlan\u0131s\u0131 i\u00e7in t\u0131klay\u0131n\u0131z) %70lik Etonol Klorofom, \u0130sopropanol 8 M LiCl (lityum Klor\u00fcr) DNA\/RNA free su Muhafaza edilen bu \u00f6rneklerden RNA izolasyonu CTAB protokol\u00fcne (Untergasser's Lab. Summer 2008) g\u00f6re derlenmi\u015ftir. Buna protokole g\u00f6re: Al\u0131nan \u00f6rnekler s\u0131v\u0131 azotta ezilir.CTAB sol\u00fcsyonuna %1\u2019lik merkaptoetanol eklenir.0,5 gr al\u0131n\u0131p \u00fczerine 600\u2026","rel":"","context":""Akademik Yaz\u0131lar\u0131m" i\u00e7inde","block_context":{"text":"Akademik Yaz\u0131lar\u0131m","link":"https:\/\/orobanche.net\/?cat=9&lang=tr"},"img":{"alt_text":"words in dictionary","src":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2022\/02\/pexels-photo-4440721.jpeg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2022\/02\/pexels-photo-4440721.jpeg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2022\/02\/pexels-photo-4440721.jpeg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2022\/02\/pexels-photo-4440721.jpeg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2022\/02\/pexels-photo-4440721.jpeg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2022\/02\/pexels-photo-4440721.jpeg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":1377,"url":"https:\/\/orobanche.net\/?p=1377&lang=tr","url_meta":{"origin":219,"position":5},"title":"Bitkilerde Lipid Peroksidasyon (MDA) Analizi","author":"graiv","date":"May\u0131s 16, 2019","format":false,"excerpt":"Malondialdehyde yani k\u0131saca MDA enzim analizi \u00f6zellikle bitkilerde stres \u00e7al\u0131\u015fmalar\u0131nda bak\u0131lmas\u0131 gereken \u00f6nemli parametrelerden bir tanesidir. Bilindi\u011fi \u00fczere stres gibi \u00e7evresel etmenlerden kaynakl\u0131 h\u00fccre membran lipidlerinin lipid peraksidasyon ile y\u0131k\u0131lmas\u0131 sonucu malondialdehyde \u00fcr\u00fcn olarak a\u00e7\u0131\u011fa \u00e7\u0131kar. MDA bu sayede lipid hasar\u0131n\u0131n ortaya \u00e7\u0131kar\u0131lmas\u0131nda \u00f6nemli bir g\u00f6sterge olarak kar\u015f\u0131m\u0131za \u00e7\u0131kar. Analiz\u2026","rel":"","context":""Akademik Yaz\u0131lar\u0131m" i\u00e7inde","block_context":{"text":"Akademik Yaz\u0131lar\u0131m","link":"https:\/\/orobanche.net\/?cat=9&lang=tr"},"img":{"alt_text":"scientist in laboratory","src":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/05\/pexels-photo-3735709.jpeg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/05\/pexels-photo-3735709.jpeg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/05\/pexels-photo-3735709.jpeg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/05\/pexels-photo-3735709.jpeg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/05\/pexels-photo-3735709.jpeg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/05\/pexels-photo-3735709.jpeg?resize=1400%2C800&ssl=1 4x"},"classes":[]}],"_links":{"self":[{"href":"https:\/\/orobanche.net\/index.php?rest_route=\/wp\/v2\/posts\/219","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/orobanche.net\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/orobanche.net\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/orobanche.net\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/orobanche.net\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=219"}],"version-history":[{"count":6,"href":"https:\/\/orobanche.net\/index.php?rest_route=\/wp\/v2\/posts\/219\/revisions"}],"predecessor-version":[{"id":1595,"href":"https:\/\/orobanche.net\/index.php?rest_route=\/wp\/v2\/posts\/219\/revisions\/1595"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/orobanche.net\/index.php?rest_route=\/wp\/v2\/media\/1594"}],"wp:attachment":[{"href":"https:\/\/orobanche.net\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=219"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/orobanche.net\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=219"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/orobanche.net\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=219"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}