{"id":250,"date":"2018-09-09T02:08:59","date_gmt":"2018-09-09T02:08:59","guid":{"rendered":"http:\/\/orobanche.net\/?p=250"},"modified":"2022-02-08T15:29:14","modified_gmt":"2022-02-08T15:29:14","slug":"molarite-hesaplama","status":"publish","type":"post","link":"https:\/\/orobanche.net\/?p=250&lang=tr","title":{"rendered":"Molarite Hesaplama"},"content":{"rendered":"

En zorland\u0131\u011f\u0131m durumlardan biriydi labaratuvarda \u00e7al\u0131\u015f\u0131rken ka\u00e7 gram katmal\u0131y\u0131m sorusu. \u0130lk zamanlar makaleri okurken ve denemeye \u00e7al\u0131\u015f\u0131rken \u201c3 mL 100 mM k-phosphate buffer (pH7.8) containing 0,5 mM EDTA-Na 2 and 0.5 mM ascorbate.\u201d g\u00f6rd\u00fc\u011f\u00fcmde korkard\u0131m. Ka\u00e7 gr kat\u0131cam ki ka\u00e7 mol olacak diye tam bir karma\u015fa gelirdi. Asl\u0131nda litre \u00e7\u00f6z\u00fcc\u00fcdeki \u00e7\u00f6z\u00fcnen mol say\u0131s\u0131na molarite denir.<\/p>\n

En basit formul\u00fc ise,<\/p>\n

Ka\u00e7 gr = kullanaca\u011f\u0131n\u0131z kimyasal\u0131n molek\u00fcl a\u011f\u0131rl\u0131\u011f\u0131 [g\/mol] X istenilen Mol [mol\/L] X Litre olarak son hacim<\/p>\n

\u00f6rne\u011fin, 100 mL  0,5 M EDTA i\u00e7in<\/p>\n

Xgr : 292.24 (molek\u00fcl a\u011f\u0131rl\u0131\u011f\u0131ndaki EDTA) X 0,0005 ( 1M : 1000mM) X 0,1 (100 mililitre i\u00e7in)<\/p>\n

X: 0,0146 gr.<\/p>\n

Bu i\u015flemler zor diyorsan\u0131z haz\u0131r web sitelerinden online olarakta hesapl\u0131yabilirsiniz.<\/p>\n

1. Sigman\u0131n web sitesi \u00fczerinden hesaplamak i\u00e7in t\u0131klay\u0131n\u0131z.<\/a><\/p>\n

2. GraphPad Prism web sitesi \u00fczerinden hesaplamak i\u00e7in t\u0131klay\u0131n\u0131z.<\/a><\/p>\n

Ben bu kadar biliyorum. Daha pek \u00e7ok site eminim ki mevcuttur. \u0130yi \u00e7al\u0131\u015fmalar.<\/p>\n","protected":false},"excerpt":{"rendered":"

En zorland\u0131\u011f\u0131m durumlardan biriydi labaratuvarda \u00e7al\u0131\u015f\u0131rken ka\u00e7 gram katmal\u0131y\u0131m sorusu. \u0130lk zamanlar makaleri okurken ve denemeye \u00e7al\u0131\u015f\u0131rken \u201c3 mL 100 mM k-phosphate buffer (pH7.8) containing 0,5 mM EDTA-Na 2 and…<\/p>\n","protected":false},"author":1,"featured_media":1591,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"_links_to":"","_links_to_target":""},"categories":[9],"tags":[34,32],"class_list":["post-250","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-genel-tr","tag-mol-hesaplama","tag-molarite"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"https:\/\/orobanche.net\/wp-content\/uploads\/2018\/09\/pexels-photo-3825457.jpeg","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p9Bqz9-42","jetpack_likes_enabled":true,"jetpack-related-posts":[{"id":248,"url":"https:\/\/orobanche.net\/?p=248&lang=tr","url_meta":{"origin":250,"position":0},"title":"Enzim Aktivite \u00d6l\u00e7\u00fcmleri \u0130\u00e7in Ezme Tamponu Haz\u0131rlanmas\u0131","author":"graiv","date":"Eyl\u00fcl 9, 2018","format":false,"excerpt":"Ba\u015flamadan \u00f6nce her zaman dedi\u011fim gibi literat\u00fcre iyice bir bak\u0131n. Baz\u0131 durumlarda farkl\u0131 bitkiler i\u00e7in farkl\u0131 ezme tamponlar\u0131 kullan\u0131lmaktad\u0131r. Genel olarak benim kullanmd\u0131\u011f\u0131m ezme tamponunun haz\u0131rlanmas\u0131n\u0131 anlataca\u011f\u0131m. SOD, CAT, APX, GPX, POD ve PPO enzim aktivitelerini belirlemede ayn\u0131 tamponu kullanabilirsiniz. \u00d6ncelikle 100mM (pH 7,5) k-fosfat tamponunuz haz\u0131r bulunmal\u0131d\u0131r. Nas\u0131l haz\u0131rland\u0131\u011f\u0131n\u0131\u2026","rel":"","context":""Akademik Yaz\u0131lar\u0131m" i\u00e7inde","block_context":{"text":"Akademik Yaz\u0131lar\u0131m","link":"https:\/\/orobanche.net\/?cat=9&lang=tr"},"img":{"alt_text":"crop chemist pouring clear liquid into fragile glassware in science center","src":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2018\/09\/pexels-photo-3825379.jpeg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2018\/09\/pexels-photo-3825379.jpeg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2018\/09\/pexels-photo-3825379.jpeg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2018\/09\/pexels-photo-3825379.jpeg?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":296,"url":"https:\/\/orobanche.net\/?p=296&lang=tr","url_meta":{"origin":250,"position":1},"title":"Agaroz Jel Elektroforezi i\u00e7in tampon haz\u0131rlamak","author":"graiv","date":"Ocak 1, 2019","format":false,"excerpt":"Jel elekroforozi, negatif y\u00fckl\u00fc olan DNA par\u00e7ac\u0131klar\u0131n\u0131n agaroz jel \u00fczerinde eksi-art\u0131 kutbu boyunca hareket etmesi ve bu sayede molek\u00fcl a\u011f\u0131rl\u0131\u011f\u0131na g\u00f6re DNA par\u00e7alar\u0131n\u0131n ayr\u0131lmas\u0131nda kullan\u0131lan bir y\u00f6ntemdir (Tabi ki jel elektroforozi sadece DNA\u2019 n\u0131n ayr\u0131lmas\u0131nda kullan\u0131lmaz protein ayr\u0131lmas\u0131 gibi farkl\u0131 kullan\u0131m ama\u00e7lar\u0131 mevcuttur).\u00a0 Jel tank\u0131nda bu hareketi sa\u011flayacak bir iyonik\u2026","rel":"","context":""Akademik Yaz\u0131lar\u0131m" i\u00e7inde","block_context":{"text":"Akademik Yaz\u0131lar\u0131m","link":"https:\/\/orobanche.net\/?cat=9&lang=tr"},"img":{"alt_text":"woman in white laboratory gown","src":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/01\/pexels-photo-3735782.jpeg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/01\/pexels-photo-3735782.jpeg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/01\/pexels-photo-3735782.jpeg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/01\/pexels-photo-3735782.jpeg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/01\/pexels-photo-3735782.jpeg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/01\/pexels-photo-3735782.jpeg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":1346,"url":"https:\/\/orobanche.net\/?p=1346&lang=tr","url_meta":{"origin":250,"position":2},"title":"Trizol Reagent Haz\u0131rlanmas\u0131","author":"graiv","date":"Mart 2, 2019","format":false,"excerpt":"\u00d6zellikle RNA izolasyonu i\u00e7in kullan\u0131l\u0131r. Trizol y\u00f6ntemi di\u011fer y\u00f6ntemler ile kar\u015f\u0131la\u015ft\u0131r\u0131ld\u0131\u011f\u0131nda KRAL d\u0131r. E\u011fer Trizol y\u00f6ntemi sizin bitki \u00f6rne\u011finizde kullan\u0131l\u0131yor ve \u00e7al\u0131\u015f\u0131yorsa kesinlikle kullan\u0131n derim. Tabi para olsa da \u0130zolasyon kitleri kullansak. Gerekli Malzemeler Sature edilmi\u015f Fenol (Folin ayrac\u0131 ile kar\u0131\u015ft\u0131rmay\u0131n. Sigma Kodu : Guanidine thiocyanate (Molek\u00fcl A\u011f\u0131rl\u0131\u011f\u0131 : 118,16\u2026","rel":"","context":""Akademik Yaz\u0131lar\u0131m" i\u00e7inde","block_context":{"text":"Akademik Yaz\u0131lar\u0131m","link":"https:\/\/orobanche.net\/?cat=9&lang=tr"},"img":{"alt_text":"crop chemist pouring clear liquid into fragile glassware in science center","src":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/03\/pexels-photo-3825379.jpeg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/03\/pexels-photo-3825379.jpeg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/03\/pexels-photo-3825379.jpeg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/03\/pexels-photo-3825379.jpeg?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":1377,"url":"https:\/\/orobanche.net\/?p=1377&lang=tr","url_meta":{"origin":250,"position":3},"title":"Bitkilerde Lipid Peroksidasyon (MDA) Analizi","author":"graiv","date":"May\u0131s 16, 2019","format":false,"excerpt":"Malondialdehyde yani k\u0131saca MDA enzim analizi \u00f6zellikle bitkilerde stres \u00e7al\u0131\u015fmalar\u0131nda bak\u0131lmas\u0131 gereken \u00f6nemli parametrelerden bir tanesidir. Bilindi\u011fi \u00fczere stres gibi \u00e7evresel etmenlerden kaynakl\u0131 h\u00fccre membran lipidlerinin lipid peraksidasyon ile y\u0131k\u0131lmas\u0131 sonucu malondialdehyde \u00fcr\u00fcn olarak a\u00e7\u0131\u011fa \u00e7\u0131kar. MDA bu sayede lipid hasar\u0131n\u0131n ortaya \u00e7\u0131kar\u0131lmas\u0131nda \u00f6nemli bir g\u00f6sterge olarak kar\u015f\u0131m\u0131za \u00e7\u0131kar. Analiz\u2026","rel":"","context":""Akademik Yaz\u0131lar\u0131m" i\u00e7inde","block_context":{"text":"Akademik Yaz\u0131lar\u0131m","link":"https:\/\/orobanche.net\/?cat=9&lang=tr"},"img":{"alt_text":"scientist in laboratory","src":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/05\/pexels-photo-3735709.jpeg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/05\/pexels-photo-3735709.jpeg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/05\/pexels-photo-3735709.jpeg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/05\/pexels-photo-3735709.jpeg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/05\/pexels-photo-3735709.jpeg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2019\/05\/pexels-photo-3735709.jpeg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":219,"url":"https:\/\/orobanche.net\/?p=219&lang=tr","url_meta":{"origin":250,"position":4},"title":"Fosfat Tamponu Nas\u0131l Haz\u0131rlan\u0131r ?","author":"graiv","date":"A\u011fustos 26, 2018","format":false,"excerpt":"\u00a0 E\u011fer literat\u00fcrde ba\u015fka bir tampon kullanman\u0131z belirtilmemi\u015f ise enzim \u00e7al\u0131\u015fmalar\u0131nda Potasyum yada Sodyum fostat tamponlar\u0131n\u0131 kullanabilirsiniz. Suda kolayl\u0131kla \u00e7\u00f6z\u00fcn\u00fcrler. Ph de\u011fi\u015fkenlik g\u00f6sterdi\u011finden dolay\u0131 ayr\u0131 ayr\u0131 haz\u0131rlay\u0131p kendiniz istedi\u011finiz Ph'y\u0131 ayarlaman\u0131z daha iyi olacakt\u0131r. Potasyum fosfat\u0131n Monobazik ve Dibazik olarak iki ayr\u0131 formlar\u0131 bulunmaktad\u0131r. Haz\u0131rlamak i\u00e7in; Dibazik potasyum fostat (K2HPO4)\u2026","rel":"","context":""Akademik Yaz\u0131lar\u0131m" i\u00e7inde","block_context":{"text":"Akademik Yaz\u0131lar\u0131m","link":"https:\/\/orobanche.net\/?cat=9&lang=tr"},"img":{"alt_text":"man in white dress shirt holding white paper","src":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2018\/08\/pexels-photo-6208927.jpeg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2018\/08\/pexels-photo-6208927.jpeg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2018\/08\/pexels-photo-6208927.jpeg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2018\/08\/pexels-photo-6208927.jpeg?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":1559,"url":"https:\/\/orobanche.net\/?p=1559&lang=tr","url_meta":{"origin":250,"position":5},"title":"Bitkilerde Prolin Analizi","author":"graiv","date":"Ocak 17, 2022","format":false,"excerpt":"Bulundurman\u0131z Gereken Malzemeler ProlinNinhidrinGlacial acetic acidEthonol (%98)Saf suEppendorf T\u00fcpBlok \u0131s\u0131t\u0131c\u0131 yada su banyosuSantrif\u00fcjmikroplateMikroplate Spektrofotometre Prolin i\u00e7eri\u011fini - \u03bcmol g-1 FW\u00a0 cinsinden hesaplayaca\u011f\u0131z. Bitkilerde bulunan prolin s\u0131cak yada so\u011fuk y\u00f6ntem kullanarak ekstrakte etmek m\u00fcmk\u00fcnd\u00fcr. 50mg taze yaprak \u00f6rne\u011fi al\u0131n\u0131r ve %70 l\u0131k 1ml ethonol:su tamponu ile havanda iyice ezildikten sonra eppendorf\u2026","rel":"","context":""Akademik Yaz\u0131lar\u0131m" i\u00e7inde","block_context":{"text":"Akademik Yaz\u0131lar\u0131m","link":"https:\/\/orobanche.net\/?cat=9&lang=tr"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/orobanche.net\/wp-content\/uploads\/2018\/08\/cropped-Screenshot_2018-08-25-mehmet-ko%C3%A7-koc35_koc-Twitter-2.png?resize=350%2C200&ssl=1","width":350,"height":200},"classes":[]}],"_links":{"self":[{"href":"https:\/\/orobanche.net\/index.php?rest_route=\/wp\/v2\/posts\/250","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/orobanche.net\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/orobanche.net\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/orobanche.net\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/orobanche.net\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=250"}],"version-history":[{"count":5,"href":"https:\/\/orobanche.net\/index.php?rest_route=\/wp\/v2\/posts\/250\/revisions"}],"predecessor-version":[{"id":1592,"href":"https:\/\/orobanche.net\/index.php?rest_route=\/wp\/v2\/posts\/250\/revisions\/1592"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/orobanche.net\/index.php?rest_route=\/wp\/v2\/media\/1591"}],"wp:attachment":[{"href":"https:\/\/orobanche.net\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=250"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/orobanche.net\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=250"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/orobanche.net\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=250"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}